نتایج جستجو برای: سودوموناس uw4
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زمینه و هدف: از آنجایی که مهار رشد در سلول های سرطانی یکی از روش های مناسب برای درمان سرطان بوده و یافتن ترکیبات ضدسرطان جدید به ویژه ترکیبات مهارکننده تکثیر، اولویت پژوهشی محسوب می گردد. امروزه در خصوص متابولیت های تولیدی جنس سودوموناس که علیه سلول های سرطان موثر می باشند، گزارش هایی وجود دارد، بنابراین در این مطالعه متابولیت های تولیدی سویه بومی سودوموناس uw4 جداسازی و اثرات ضدمیکروبی و ضدسرط...
The plant growth-promoting bacterium (PGPB) Pseudomonas sp. UW4, previously isolated from the rhizosphere of common reeds growing on the campus of the University of Waterloo, promotes plant growth in the presence of different environmental stresses, such as flooding, high concentrations of salt, cold, heavy metals, drought and phytopathogens. In this work, the genome sequence of UW4 was obtaine...
A typical plant response to any biotic or abiotic stress, including cadmium (Cd), involves increased ethylene synthesis, which causes senescence of the affected plant part. Stressed plants can experience reduced ethylene and improved growth if they are inoculated with bacteria that have the enzyme ACC deaminase, which metabolizes the ethylene precursor ACC (1-aminocyclopropane-1-carboxylate). W...
The growth of transgenic canola (Brassica napus) expressing a gene for the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase was compared to non-transformed canola exposed to flooding and elevated soil Ni concentration, in situ. In addition, the ability of the plant growth-promoting bacterium Pseudomonas putida UW4, which also expresses ACC deaminase, to facilitate the growth of non-tran...
The study aimed at identifying and purifying cytosolic glutathione transferase isoforms expressed in Pseudomonas sp. UW4. Search UniProt (https://www.uniprot.org/uniprot/), has indicated that there were 20 genes encoding putative transferases for the microorganism. molecular weights of ranged from 17.6 to 34.06 kDa. SDS-polyacrylamide gel electrophoresis revealed GST purified using Sulfobromoph...
Background: Breast cancer is a malignant proliferation of epithelial cells that lining the ducts or lobules of the breast. It is the second common cancer, after lung cancer in women. Since growth inhibition is an important strategy in cancer treatment, many attempts are in program to find new apoptotic inducer agents. Today there is some reports about effect of metabolites of Pseudomonas on can...
Introdution: Regarding to the development the problems of systemic toxicity and drug resistance in cancer chemotherapy, the continuing discovery of new bioactive compounds and anticancer agents is very necessary. Therefore, in this study, we checked the anticancer activity of native Pseudomonas sp. UW4 metabolite to find new compound. Methods: This experimental study was performed in Shahrekor...
The enzyme 1-aminocyclopropane-1-carboxylate deaminase (ACCD) converts ACC, the precursor of the plant hormone ethylene, to alpha-ketobutyrate and ammonium. This enzyme has been identified in soil bacteria and has been proposed to play a key role in microbe-plant association. A soluble recombinant ACCD from Pseudomonas putida UW4 of molecular weight 41 kDa has been cloned, expressed, and purifi...
مقدمه: آلفانفتول، هیدروکربن آروماتیک دوحلقهای، جهشزای بالقوه و ترکیبی سمی برای موجودات است. حذف کامل آلایندهها و تولیدنشدن آلایندههای ثانویه، سهولت در اجرا و بینیازی از ابزارها و مواد شیمیایی گرانقیمت از دلایل اهمیت روشهای اصلاح زیستی نسبت به فناوریهای فیزیکوشیمیایی برای پاکسازی منطقه آلوده هستند. مواد و روشها: ابتدا محیط پایه نمکی (BSM) تهیه و آلفانفتول، منبع کربن برای رشد باکتری...
Pseudomonas fluorescens UM270 is a rhizosphere-colonizing bacterium that produces multiple diffusible and volatile compounds involved in plant growth-promoting activities. Strain UM270 exhibits excellent biocontrol capacities against diverse fungal pathogens . In a previous study, the general UM270 genome characteristics were published. Here, we report a deeper analysis of its gene content and ...
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